Two summers ago, I was entrusted to lead my own research project in Dr. Entcheva’s Cardiac Optogenetics and Optical Imaging Lab. Tasked to design a three-dimensional “spheroid” of cells, I was to begin by exploring methods to construct and handle the self-assembling structures. Both excited and apprehensive at the prospect of developing my own project, I could not wait to get started.
Going in, I was aware the process wouldn’t be as straightforward as completing another class assignment. I was basically starting a new development from scratch. I was the scientist. I was the explorer. I was the pioneer. Indeed, after the first iteration of testing, I noticed the cellular structures were fragile and easily broken. Despite my initial attempts at gentler handling methods, I met with the same results. Eventually, I realized the problem did not originate from a single point-of-failure. I thought critically about the different variables I could adjust to achieve a more stable structure. Only after additional attempts employing different seeding densities, culturing conditions, and protocol adjustments was I able to produce a more durable “spheroid” that propelled this stage of my project to completion.
I now realize the delight of tackling challenges in new fields with little guidance. Driven by my own curiosity of the unknown and my determination to succeed, I was especially excited to have developed my own protocol for generating three-dimensional cellular structures. As I have learned over the past year, research is journey that challenges one’s drive and determination. A student with these attributes will surely be set up for success in his or her research endeavors.